Choice

Missed this blog so badly, I am so engaged in lab work recently. Yesterday we had a meeting with Dr E and masters. We all discussed on the progress, which we had been dragged so far behind as compared to others' progress. We had failed in cloning, were gone through troubleshooting by using another badge of Laix plate which likewise couldn't give blue colonies, checked back ligation products which sadly indicates that the ligation of vector and insert did not take place. We also doubt on the competent cells' quality as they were produced since 2008. As a whole story tells here is that we are not confident of our result and skills anymore. Dr E and masters, undoubtedly were rather disappointed, although they did not really show the face to us. Dr E is so kind, that he manages to comfort and give neutral comments which makes us feel warm at the particular moments. Sooner after that, back to masters, they are the ones throwing all bad words and push us to reality. This is them, this is our lab,our work. After all, we are still the one be responsible to our FYP.

After the meeting, we had a small sub-meet with Stella, which she said she would help us to do the project now. First heard of this, we suppose this act is to the extent of their frustrated act. They could not bear it anymore, as they themselves are working pretty fine to get results with no errors, and we just out of ideas, could not generate data in the so long trial period. What would be the problem? And now, we have her to help us, what should we feel? Happy or sad? If she manages to get results, means our human error is the factor that cause the failure of us all the time; in contrary, if she could not get results, we will be discouraged and disappoint. I have ever experienced it, even Kherlee helped me, I still have one naughty gene, which insist not showing up all the while.

Are we too weak? Why are we so miserable? My roommate, CH she will finish her project soon in one more week, two more steps. I always think of how nice if we choose another titles, we could have steadily advanced through stages to get results already, like her, like Dr Leaw's lab.Next time, the advice to juniors of taking FYP, please choose your lab, your masters, rather than your supervisor and the titles. Titles of interest should come in last, if the masters nice, your chances of getting results and completing projects would be doubly high. Don't be so naive to choose titles first, any titles could just grow your interest if you do it smoothly~!